| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1147-1 | ||||
Resumo:Although Enterococcus spp are members of the gut microbiota, several species can cause disease in humans. E. faecalis (Efs) and E. faecium (Efm) are the most studied among them due to their prevalence in healthcare-associated infections (HAIs). However, other species have emerged as potential pathogens. Pets are present in the households of many families. So, the close contact between animals and their owners may allow for interspecies bacterial transmission, including microbiota bacteria. Therefore, this study aims to evaluate the antimicrobial resistance and virulence patterns of NEfs-NEfm of canine origin. One hundred fifteen isolates were obtained from rectal swab of dogs between 2015 and 2017 in Rio de Janeiro. By MALDI-TOF, the following species were identified: E. gallinarum (n= 48), E. casseliflavus (n: 17), E. hirae (n: 17), E. avium (n: 14), E. canintestini (n: 9), E. raffinosus (n: 9) and E. canis (n: 1). Disk diffusion (16 antimicrobial agents), agar gradient diffusion (vancomycin) and PCR (tetracycline, macrolide, vancomycin, and aminoglycoside genes) were performed to investigate the antimicrobial resistance pattern. The virulence pattern was investigated by PCR for genes asa1, cylA, gelE, esp e hyl. The highest resistance frequencies were observed for rifampicin (38%), tetracycline (21%) and erythromycin (16%). Only E. gallinarum isolates exhibited high-level of aminoglycoside (8%) and vancomycin resistance (0.8%; MIC > 512 µg/mL). Ampicillin resistance was observed in the most E. raffinosus isolates (55%). E. raffinosus (56%), E. canintestini (22%) and E. gallinarum (13%) isolates were multidrug resistant. The resistance genes detected were ant(6)-Ia (6%), erm(B) (6%), van operon (2%), tet(L) (14%) and tet(M) (22%). Few isolates carried some of the virulence genes investigated. The esp gene was the most frequently observed: E. avium (42%), E. canintestini (44%) and E. raffinosus (33%). Even though only some isolates have been multidrug-resistant, dogs may be source of several genes that may interfere in the effectiveness of treatment of enterococcal infections. In relation the virulence, the frequency of esp in some species request studies to identify its role. Palavras-chave: Enterococcus spp., antimicrobial resistance, virulence, dog Agência de fomento:CAPES, CNPq | |||||